Protocols

Provided by Wayne Parrott
University of Georgia Chapel, Herty Dr, Athens, GA 30602, USA

Maize Mesophyll Protoplast Protocol

A Unified Agrobacterium-Mediated Transformation Protocol for Alfalfa (Medicago sativa L.) and Medicago truncatula

Qingzhen Jiang, Chunxiang Fu, and Zeng-Yu Wang
https://link.springer.com/protocol/10.1007/978-1-4939-8778-8_11

Protocol Addendum

Preparation of AGL-1 Electrocompetent Agrobacterium Cells

Provided by Joyce Van Eck
Boyce Thompson Institute, 533 Tower Rd, Ithaca, NY 14853, USA

Glycerol Stock Production for Agrobacterium

Provided by Joyce Van Eck
Boyce Thompson Institute, 533 Tower Rd, Ithaca, NY 14853, USA

Agrobacterium transformation by electroporation

Provided by Wayne Parrott

University of Georgia Chapel, Herty Dr, Athens, GA 30602, USA

Frozen Bacterial Stocks_Gelvin Lab Protocol

Provided by Veena Veena

Donald Danforth Plant Science Center, 975 N Warson Rd, Olivette, MO 63132, USA

Transformation of Agrobacterium strains by Electroporation

Provided by Keunsub Lee

Iowa State University, Ames, IA 50011, USA

Storage of competent cells for Agrobacterium transformation

Provided by Keunsub Lee

Iowa State University, Ames, IA 50011, USA

Agrobacterium-mediated Rice Transformation from Mature Seeds

Provided by Albert Kausch 

University of Rhode Island, South Kingston, RI 02881, USA

Additional Resources

Masterclass Series

A collection of videos on plant transformation and gene engineering

Interactive Series for Plant Transformation Protocols

A guide through protocols by the author(s)