Interactive Series for Plant Transformation Protocols

Plant transformation protocols are often challenging to successfully reproduce. Small differences in experiments can result in failed transformations. This series will feature interactive talks from experts. Basic steps of transformation will be covered, do’s and don’ts, and helpful tips.

Optimized Sorghum Leaf Transformation

Sorghum can be difficult to transform due to explant availability, phenolic compound accumulation, and genotype dependence. This protocol has been successfully demonstrated in 13 sorghum genotypes. Seedling leaf whorl is transformed via Agrobacterium with the support of morphogenic transcription factors. 

Speakers will guide attendees through this protocol step-by-step, including practical tips and pitfalls to avoid. The “why” behind each step will also be discussed. Attendees are expected to read the protocol before the event and come prepared with questions. Expect lots of discussion in this interactive webinar! 

Speakers

Mercy Azanu

PhD Student
Iowa State University

Faizo Kasule

PhD Student
Iowa State University 

Dibyajyoti Pramanik

Postdoc Research Associate
Iowa State University